Douglas Mahoney

Over the past decade, immunotherapy has emerged as a “fourth pillar” of cancer therapy. Our lab is developing novel virus- and T cell-based immunotherapies for treating pediatric solid tumours and other cancers. We use a variety of standard and cutting-edge multidisciplinary techniques, including intravital microscopy and other in vivo imaging modalities; flow cytometry and other standard immunology methods; high-throughput drug, RNAi and CRISPR screening; genetic engineering of viruses, cancer and T cells; and next-generation sequencing. Our primary model system is the mouse. We use syngeneic murine models of pediatric sarcoma, neuroblastoma and adult breast cancer, including novel autochthonous sarcoma models currently being developed. Our research encompasses the study of both the cancer and its environment, including secondary lymphoid organs and the gut microbiome.