High-Throughput Spatial Omics

GeoMx Digital Spatial Profiler (DSP): the most flexible and robust spatial multi-omic platform for analysis of formalin-fixed paraffin-embedded (FFPE) and fresh frozen (FF) tissue sections. GeoMx is the only spatial biology platform that non-destructively profiles the expression of RNA and protein from distinct tissue compartments and cell populations with an automated and scalable workflow that integrates with standard histology staining. It uses fluorescent imaging reagents to visualize tissue and oligo-tagged reagents for expression analysis. After selecting regions of interest (ROI), DNA oligos are released, and collected in a 96-well plate. Expressions levels are readout using Next-Generation Sequencing (NGS) or nCounter and analyzed using the DSP interactive software.

Spatial Organ Atlas: a publically available resource for defining the molecular anatomy of tissues. Using a whole transcriptome approach to generate spatially resolved organ maps the Spatial Organ Atlas presents morphology-driven data at the molecular level within the context of each organ-specific substructure. 

Visit Nanostring's Spatial Orgain Atlas

CosMx Spatial Molecular Imager (SMI): the first high-plex in situ analysis platform to provide spatial multiomics with FFPE and FF tissue samples at cellular and subcellular resolution. CosMx SMI enables rapid quantification and visualization of up to 1,000 RNA and 64 validated protein analytes. It is the flexible, spatial single-cell imaging platform that will drive deeper insights for cell atlasing, tissue phenotyping, cell-cell interactions, cellular processes, and biomarker discovery.

What is the difference between GeoMx and CosMx?

GeoMx can be used to investigate up to the whole transcriptome of 18,000+ targets and >100 antibodies on the same slide. CosMx resolves RNA and protein expression at single molecule, subcellular resolution in situ.

Can we perform label-free and multi-fluorescence image analysis?

Image analysis is not doable since the Nanostring team deprecated the functions that can export images. This limitation won't allow users to (re)segment the images with their methodologies on their local machine. For more information, please visit here.

Xenium In Situ: it enables high-throughput subcellular mapping of 100s of RNA targets, revealing new insights into cellular structure and function. Tissue sections are placed on Xenium slides and treated to access RNA for labeling with circularizable DNA probes. Probe ligation generates a circular DNA specific to target transcripts followed by enzymatic amplification to create a target for fluorescent probe hybridization. The slide is then put into the Xenium Analyzer, where it undergoes rounds of fluorescent probe hybridization, imaging, and removal. This generates an optical signature that reveals the identity of the RNA at a location within each cell of a tissue.

Xenium panels are carefully designed and curated, incorporating a data-driven approach that combines expert input with years of single-cell experience. Customization is fast and easy, letting you add to existing panels or order your own standalone panels specifically tailored to power your research.

• Human Breast, Brain, Lung, and Multi-Tissue/Cancer Gene Expression Panel
• Mouse Brain Gene Expression Panel and Xenium Mouse Tissue Atlassing Panel

Visium Spatial Gene Expression: a next-generation molecular profiling solution for classifying tissue based on total mRNA. Map the whole transcriptome with morphological context in FFPE or fresh frozen tissues to discover novel insights into normal development, disease pathology, and clinical translational research. Visium maps gene expression in complex tissue samples using poly(A) capture and spatial barcoding technology for library preparation. mRNA-binding oligonucleotides bind to capture oligos on tissue sections, and reverse transcription generates a cDNA library with complements of spatial barcodes that preserve spatial information.

Visium CytAssist: a compact, benchtop instrument that enables the transfer of transcriptomic probes from standard glass slides to Visium slides, enabling spatial profiling insights to be gained from even more samples. Compatible with hematoxylin and eosin (H&E)- or immunofluorescence (IF)-stained tissue sections, CytAssist allows pre-sectioned tissues to be used for the Visium workflow. You can further maximize your Visium experiments by screening tissue sections using standard histological techniques to find biologically significant sections and then precisely align those sections within the Visium slide Capture Area using CytAssist.