Next-Gen Sequencing

We use the latest technology from Illumina for sequencing, and store your data on our secure private servers. You can choose to have us prepare your libraries, or prepare them yourself for sequencing on our instruments.

Consultations

Illumina offers a wide range of kits with varying data output and read length to match your sequencing needs. To get an idea of what instruments we offer and what sequencing parameters are available, see the tables below, and then book a consultation to discuss your project with us to determine which sequencing kit is best suited for your project goals.

Illumina NovaSeq 6000

Illumina's latest high-throughput sequencer, the NovaSeq 6000, can generate up to 3000 gigabases of sequence from 10 billion reads per flow cell, and is capable of running two flow cells simultaneously. Paired-end sequencing is available up to 2 x 250 bp read lengths.

 

See the table below for the sequencing kits available from Illumina and their common applications.

Illumina NovaSeq 6000, image courtesy of Illumina, Inc.
Flow Cell Type Number of Cycles (bp) Number of Read Pairs (M) Data Output (Gbp)
SP 100 800 80
  200 800 167
  300 800 250
  500 800 400
S1 100 1,600 167
  200 1,600 333
  300 1,600 500
S2 100 4,100 417
  200 4,100 833
  300 4,100 1,250
S4 200 10,000 2,000
  300 10,000 3,000

 

 

Common applications include but are not limited to:

  • whole-genome sequencing
  • whole-exome sequencing
  • whole-transcriptome sequencing
  • targeted DNA sequencing
  • methylation sequencing
  • gene espression profiling with mRNA-Seq
  • de novo sequencing
  • ChIP-seq
  • population studies
  • metagenomics

Illumina NextSeq 500

The NextSeq 500 is a high-throughput instrument capable of generating up to 120 GB from 400 million reads and 2 x 150 bp read lengths.

Illumina NextSeq, image courtesy of Illumina, Inc.
Sequencing Kit Cycles (bp) Number of Reads (M) Data Output (Gbp) Application
v2.5 150 cycle mid output

2 x 75, or

1 x 150

 130 20 Whole transcriptome, mRNA-seq, exome, ChIP-seq
v2.5 300 cycle mid output

2 x 150, or

1 x 300

 130 40 Targeted gene panels, exome
v2.5 75 cycle high output

1 x 75

 

 400 30 Gene expression profiling
v2.5 150 cycle high output

2 x 75, or

1 x 150

 400 60 Whole transcriptome, mRNA-seq, exome
v2.5 300 cycle high output

2 x 150, or

1 x 300

 400 120 Whole genome, de novo, exome

 

Illumina MiSeq

A cost-effective sequencing tool, the MiSeq can produce up to 15 GB of data from 25 million sequencing reads per run, with a maxim read length of 2 x 300 bp.

Illumina MiSeq, image courtesy of Illumina, Inc.
Sequencing Kit Cycles (bp) Number of Reads (M) Data Output (Gbp)
v2 300 cycle nano

2 x 150, or

1 x 300

 1 0.3
v2 500 cycle nano

2 x 250

 

 1 0.5
v2 300 cycle micro

2 x 150, or

1 x 300

 4 0.5
v2 50 cycle

2 x 25, or

1 x 50

 15 0.8
v2 300 cycle

2 x 150, or

1 x 300

 15 5
v2 500 cycle

2 x 250

 

 15 8.5
v3 150 cycle

2 x 75, or

1 x 150

 25 3
v3 600 cycle

2 x 300

 

 25* 15

 

*10-15M reads for low diversity libraries, i.e. 16S rRNA metagenomics

 

Common applications include but are not limited to:

    • 16S metagenomics
    • amplicon sequencing and targeted gene panels
    • de novo small-genome sequencing
    • ChIP-seq
    • other small sequencing projects