Next-Gen Sequencing

We use the latest technology from Illumina for sequencing, and store your data on our secure private servers. You can choose to have us prepare your libraries, or prepare them yourself for sequencing on our instruments.

Consultations

Illumina offers a wide range of kits with varying data output and read length to match your sequencing needs. To get an idea of what instruments we offer and what sequencing parameters are available, see the tables below, and then book a consultation to discuss your project with us to determine which sequencing kit is best suited for your project goals.

Illumina NovaSeq 6000

The NovaSeq 6000 can generate up to 3000 gigabases of sequence from 10 billion reads per flow cell, and is capable of running two flow cells simultaneously. Paired-end sequencing is available up to 2 x 250 bp read lengths.

 

See the table below for the sequencing kits available from Illumina and their common applications.

Illumina NovaSeq 6000, image courtesy of Illumina, Inc.
Flow Cell Type Number of Cycles (bp) Number of Read Pairs (M) Data Output (Gbp)
SP 100 800 80
  200 800 167
  300 800 250
  500 800 400
S1 100 1,600 167
  200 1,600 333
  300 1,600 500
S2 100 4,100 417
  200 4,100 833
  300 4,100 1,250
S4 200 10,000 2,000
  300 10,000 3,000

 

 

Common applications include but are not limited to:

  • whole-genome sequencing
  • whole-exome sequencing
  • whole-transcriptome sequencing
  • targeted DNA sequencing
  • methylation sequencing
  • gene espression profiling with mRNA-Seq
  • de novo sequencing
  • ChIP-seq
  • population studies
  • metagenomics

*NEW* Illumina NextSeq 2000

The NextSeq 2000 can generate 10 - 360 Gb of sequence data from 100 million - 2.4 billion reads per flow cell, making it well-suited for small whole genome sequencing, exome sequencing, RNA-seq, 16S RNA profiling, and smaller single-cell RNA-seq projects.

Image courtesy of Illumina, Inc.
Sequencing KitCycles (bp)Number of Reads (M)Data Output (Gbp)
P1 100 cycle2 x 50, or 1 x 10010010
P1 300 cycle2 x 150, or 1 x 30010030
P1 600 cycle2 x 30010060
P2 v2 100 cycle2 x 50, or 1 x 10040040
P2 v3 200 cycle2 x 100, or 1 x 20040080
P2 v3 300 cycle2 x 150, or 1 x 300400120
P2 600 cycle2 x 300300180
P3 50 cycle1 x 50120060
P3 100 cycle2 x 50, or 1 x 1001200120
P3 200 cycle2 x 100, or 1 x 2001200240
P3 300 cycle2 x 150, or 1 x 3001200360

 

Illumina MiSeq

A cost-effective sequencing tool, the MiSeq can produce up to 15 GB of data from 25 million sequencing reads per run, with a maxim read length of 2 x 300 bp.

Illumina MiSeq, image courtesy of Illumina, Inc.
Sequencing Kit Cycles (bp) Number of Reads (M) Data Output (Gbp)
v2 300 cycle nano

2 x 150, or

1 x 300

 1 0.3
v2 500 cycle nano

2 x 250

 

 1 0.5
v2 300 cycle micro

2 x 150, or

1 x 300

 4 0.5
v2 50 cycle

2 x 25, or

1 x 50

 15 0.8
v2 300 cycle

2 x 150, or

1 x 300

 15 5
v2 500 cycle

2 x 250

 

 15 8.5
v3 150 cycle

2 x 75, or

1 x 150

 25 3
v3 600 cycle

2 x 300

 

 25* 15

 

*10-15M reads for low diversity libraries, i.e. 16S rRNA metagenomics

 

Common applications include but are not limited to:

  • 16S metagenomics
  • amplicon sequencing and targeted gene panels
  • de novo small-genome sequencing
  • ChIP-seq
  • other small sequencing projects