High Throughput Microscopes
Fully automated systems for high content imaging of well plates, dishes, and slides.
Olympus VS110 Slide Scanner (5 Slides)
Imaging Modes
Brightfield
Widefield: DAPI, FITC, TRITC, Cy5
Objectives
2x/0.08 air; 10x/0.4 air; 20x/0.75 air; 40x/0.95 air; 60x/1.35 oil
Filters
DAPI ex 387/11 nm – em 440/40 nm; FITC ex 485/20 nm – em 525/30 nm; TRITC ex 560/25 nm – em 607/36 nm; Cy5 ex 650/13 nm – em 684/24 nm
Cameras
Olympus XC10 and XM10
Software
VS-ASW-S5 (V2.9) with Batch Converter
CellSens (Offline Analysis)
Free OlyVIA viewer (Windows Only)
Open Source viewer and analysis QuPath (Windows, Mac, Linux)
Standard Operating Procedure
Download Olympus_VS110_sop
Olympus VS120 Slide Scanner (100 Slides)
Imaging Modes
Brightfield
Widefield:
Multiband - DAPI/FITC/TRITC/Cy5 or CFP/YFP/RFP
Singleband - DAPI, FITC, TRITC, Cy5
Filters
see SOP or Full Specs links below
Objectives
2x/0.08 air; 10x/0.4 air; 20x/0.75 air; 40x/0.95 air
Cameras
Allied Vision Pike F-505C color CCD camera (8-bit Brightfield)
Hamamatsu Orca Flash 4.0 sCMOS monochrome (16-bit Fluorescence)
Software
VS-ASW-L100 (V2.9) with Batch Converter
CellSens (Offline Analysis)
Free OlyVIA viewer (Windows Only)
Open Source viewer and analysis QuPath (Windows, Mac, Linux)
Standard Operating Procedure
Download Olympus_VS120_sop
ImageXpress Micro XLS High-Content Analysis System
Imaging Modes and Features
5-channel fluorescence imaging: DAPI, FITC, Cy3, TxRed, Cy5, Cy7 (on request)
Full incubator environmental control: Temperature range 30-40°C ± 0.5°C, premixed gas
Compatible with slides, dishes, well plates
High speed 690 nm laser autofocusing
Real-Time and/or Post Acquisition Image Processing & Analysis
Objectives
4x/0.2 NA; 10x/0.3 NA; 10x/0.5 NA; 20x/0.45 NA; 40x/0.60 NA; or any Nikon lens!
Light source
Excelitas X-Cite Xylis Broad Spectrum LED Illumination System
DAPI to Cy7 (380-770nm)
Filters
DAPI ex 387/11 – em 447/60; FITC ex 482/35 – em 536/40; Cy3 ex 531/40 – em 593/40; Texas Red ex 562/40 – em 624/40; Cy5 ex 628/40 – em 692/40; Cy7 ex 710/75 - em 810/90
Camera
Photometrics CoolSNAP HQ monochrome CCD
Software
MetaXpress High-Content Image Acquisition and Analysis
Standard Operating Procedure
Download MolDev_IXM_sop