STELLARIS 8 STED

Leica STELLARIS 8 STED FALCON DIVE

Stimulated Emission and Depletion Super-resolution | Fluorescence Lifetime Imaging | Multiphoton | Fluorescence Correlation Spectroscopy | Near-IR Detection

STED Super-Resolution with TauSTED Xtend

STED Super-Resolution with TauSTED Xtend

Image beyond the diffraction limit in X, Y, and Z. TauSTED Xtend combines lifetime-based information resulting in a significant gain in resolution at lower depletion powers, allowing multicolor live imaging capabilities.

FALCON Fluorescence Life Imaging Microscopy (FLIM)

FALCON Fluorescence Life Imaging Microscopy (FLIM)

FALCON (FAst Lifetime CONtrast) is a fully integrated FLIM solution 

For more info on applications click below:

FLIM

DIVE Multicolour Multiphoton

DIVE Multicolour Multiphoton

Fully tuneable emission detection between 380 – 800 nm.
Combine with TauSense for lifetime-based separation and metabolic imaging of endogenous fluorophores (e.g. NADH or FAD).

Please contact HBIAMP for more information.

Leica STELLARIS 8 - STED FALCON DIVE

Specifications and Equipment

 

Imaging Modes and Features

  • Brightfield Transmitted Light
    • Phase Contrast
    • Polarisation
    • DIC
  • Widefield Fluorescence
    • Eyepiece: DAPI, FITC, CY3, CY5 filters.
    • Camera: Leica K5 sCMOS (4.2 megapixel 40 fps).
  • Confocal Laser Scanning
    • 8k resolution X2Y linear Galvo scanner (with scan-rotation).
    • 2k resolution 12kHz Resonant scanner (with scan-rotation).
    • Super Z-Galvo stage (fast Z-stack).
      Acquisition: tiling & stitching, z-stack, multiposition, timelapse, lambda scans, unmixing
  • Stimulated Emission and Depletion (STED) Super-Resolution microscopy
    • STED 3D module: Tunable super-resolution in x, y, and z.
    • TauSTED Xtend: Utilising fluorescence lifetime to further increase resolution while decreasing depletion laser power.
  • FALCON (FAst Lifetime CONtrast) Fluorescence Lifetime Imaging Microscopy (FLIM)
    • Fast FLIM (at confocal speed) in confocal and STED imaging modes.
    • FLIM analysis tools: Exponential fitting, FLIM phasor analysis, pattern fitting and FLIM-FRET analyzer.
    • For applications and more info click here.
  • Fluorescence Correlation Spectroscopy
    • Fluorescence correlation and crosscorrelation and lifetime
      spectroscopy (FCS/FLCS) measurements. 
  • Multiphoton Microscopy
    • Deep volumetric imaging using near-IR excitation.
    • Variable Beam Expander: Optimise between depth and resolution.
    • 4TUNE HyD NDD x 2: Fully spectrally tunable detectors.

    • Combine with TauSense for lifetime contrast and separation of endogenous fluorphores (e.g. NADH and FAD).

       

Tunable detection channels (Confocal and STED modes)

  • Confocal/STED (400 - 850 nm detection window)
    • HyD S detector x 2: Si-based, all round performance, high-efficiency in blue/green
    • HyD X detector x 2: Specialized for FLIM, FCS and Tau-STED in the visible up to far-red
    • HyD R detector x 1: Specialized for FLIM and FCS in the NIR range
  • Multiphoton DIVE (380 - 800 nm detection window)
    • 4TUNE HyD Non-Descanned x 2: Fully spectrally tunable detectors.
  • Miscellaneous
    • Avalanche Photodiode x 1: For Anisotropy etc.
    • Transmitted light detector (PMT)

 

 

 

Lasers
Excitation

  • 405 nm pulsed diode laser
  • Tunable White Light Laser: 440- 790 nm tuning range (1 nm increments) 1- 80 MHz Repitition Rates. Up to 8 simultaneous excitation lines possible
  • Coherent Chameleon Ultra I: 690 - 1040 nm tuning range

Depletion

  • 589 nm (Pulsed), 660 nm (CW ), and 775 nm (Pulsed) diode lasers

Objectives

Phase

  • 5x/0.12NA Dry
  • 10x/0.32NA Dry

Widefield, Confocal, General

  • 5x/0.15NA Dry
  • 10x/0.40NA Dry
  • 20x/0.75NA Dry
  • 20x/0.75NA Multi-Immersion
  • 25x/0.95NA Water
  • 40x/1.10NA Water (Motorised correction collar)
  • 63x/1.40NA Oil

STED

  • 86x/1.20NA Water (Motorised correction collar)
  • 93x/1.30NA Glycerol (Motorised correction collar)
  • 100x/1.40NA Oil

Software
Leica STELLARIS Control Software - LASX Modules

  • 3D Visualisation
  • Lightning Expert + Lightning STED Expert
  • TauSTED XTend
  • Navigator Expert
  • Dye Finder
  • FRAP Zoomer
  • Assay Editor
  • Microlab
  • Electrophysiology
  • Rare Event Detection
  • Aivia Digital Signal Enhancement (DSE)
  • FALCON FLIM Module with Phasor FLIM Analysis

LASX Offline Analysis

  • 3D Visualisation and Analysis
  • FLIM
  • FCS
  • Lightning Process
  • Aivia Go