Confocal Microscopes - Fluorescence Lifetime Imaging (FLIM)
These microscopes are designed for imaging fluorescent samples with high resolution, high contrast, high sensitivity, 3D optical/Z-sectioning.
Additionally, they also feature fluorescence lifetime imaging (FLIM) capabilities, an imaging technique based on differences in exponential decay rates of fluorescent probes from a sample featuring video-rate speeds with pixel-by-pixel quantification.
Leica STELLARIS 8 - STED FALCON DIVE
Features:
Stimulated Emission and Depletion (STED) Super-resolution, Fluorescence Lifetime Imaging (FLIM), Multiphoton, White Light Laser, NIR detection, Multiplexing and more...
Fully featured Super-Resolution, Fluorescence Lifetime and Multiphoton microscope. Details at the link.
Leica SP8 FALCON
Features:
Fluorescence Lifetime Imaging Microscopy (FLIM), White Light Laser, Multiplexing
Imaging Modes
Inverted DMI8 Platform
Brightfield Transmitted Light and DIC (eyepiece)
Widefield Fluorescence (eyepiece): DAPI, FITC, Rhodamine
Confocal Laser Scanning: X2Y linear Galvo scanner and 12kHz Resonant scanner (both with scan rotation)
Super Z-Galvo stage (fast Z-stack)
Acquisition: tiling & stitching, z-stack, multiposition, timelapse, lambda scans, unmixing
Detectors (5 x tuneable channels)
Variable filters 380 - 800 nm
1 x Low dark current Hamamatsu PMT detectors
2 x High sensitivity hybrid detectors (photon counting mode available)
2 x Cooled high sensitivity, single molecule detection (SMD) hybrid detectors (optimised for FLIM)
1 x Fixed Transmitted light detector
Lasers
405 nm diode laser, 440 nm pulsed diode laser
Tunable White Light Laser 470 - 670 nm (1 nm increments) 10 - 80 MHz Rep Rate
Objectives
2.5x/0.07NA dry; 5x/0.15NA dry; 10x/0.40 NA dry; 20x/0.8 NA dry; 25x/0.95 NA water; 63x/1.40NA oil
Software
Leica Application Suite X (download LASX Office Viewer)
FALCON FLIM Module with Phasor FLIM Analysis
Standard Operating Procedure
Download Leica_TCS_SP8_FALCON_SOP