Multiphoton Microscope

Deep 3D infrared-based imaging of fluorescence and second harmonic generation in ex-vivo and in-vivo samples.

Nikon A1R-MP Multiphoton Microscope

A1

Imaging Mode and Features
Widefield Fluorescence: DAPI, GFP, and DSRed
Confocal/Multiphoton Laser Scanning: Galvo-Galvo or high speed Galvo-Resonant scan modes 
Acquisition: z-stack, tiling & stitching, spectral unmixing, multipoint, timelapse

Detection

Visible Laser Confocal Scanning
4 channel standard PMT detectors 
32 channel spectral Multi-Anode detector: 400-750 nm visible range; 2.5/5/10 nm variable resolution

Multiphoton Laser Scanning
Ti:Sapphire tunable femtosecond pulsed laser 680-1040 nm
4 channel standard PMT detectors
4 channel High sensitivity NDD (Non Descanned) detectors
32 channel spectral Multi-Anode detecto: 400-650 nm with IR laser; 2.5/5/10 nm variable resolution

Objectives 
10x/0.45 NA air; 20x/0.75 NA multi-immersion; 60x/1.27 NA water; 16x/0.80 NA water; 25x/1.10 NA water

Lasers 
405 nm (DAPI); 457, 477, 488, 514 nm (GFP, YFP, FITC, Alexa488); 561 nm (Cy3); 640 nm (Cy5, Alexa 647)
Coherent Chameleon Ultra I Ti:Sapphire tunable femtosecond pulsed laser (680 - 1040 nm)

Common filter combinations 
Blue, Green, Red, Far Red: 450/50 - 525/50 - 600/50 - 685/70 nm

Software
NIS-Elements AR (download NIS-Elements viewer)

Standard Operating Procedure
Download Nikon_A1mp_sop