Multiphoton Microscope

Description

A completely modular multiphoton imaging platform that adapts to a wide range of experimental requirements. Featuring five axes of movement including a rotating body, which allows the microscope to rotate around the sample. Equipped with fast resonant scanners and sensitive detectors.

Imaging Mode and Features

Widefield Fluorescence

• DAPI and GFP LEDs and filter sets.
• 5MP Monochrome CMOS Kiralux Thorlabs camera.

Laser Scanning 

• 450-1100 nm wavelength range.
• Dual scan paths: Galvo-Galvo (GG) and Galvo-Resonant (GR).
• (GG): Custom scan geometries and user-defined ROIs for photo stimulation.
• (GR): 12 kHz: 4.4 fps (2048 x 2048 Pixels); 45 fps (512 x 512 Pixels); 600 fps (512 x 32 Pixels).

Acquisition

• Deep volumetric imaging (Z-stack), tiling, multipoint, time series.

Detection

• 2 x High sensitivity GaAsP PMTs.

Objectives 

• Multiphoton: Nikon LWD 3.0 mm 16x/0.80 NA water.
• Multiphoton: Nikon Apo LWD 2.0 mm 25x/1.10 NA water.
• Can accept any Infinity-corrected objectives with M34 x 1.0, M32 x 0.75, M25 x 0.75, or RMS threads (with an adapter).

Lasers

Coherent Chameleon Ultra II Ti:Sapphire tunable femtosecond pulsed laser (680 - 1080 nm).

Additional Features

• Piezo objective scanner: Enables objective positioning and Z-Stack acquisition with Resolution ~1 nm.
• Rotation: 0° to 90° around the Sample.

Software
ThorImage®LS