Multiphoton Microscope

Deep 3D near-infrared imaging of two-photon fluorescence and second harmonic generation in live in-vivo and ex-vivo samples.

Thorlabs BERGAMO® II Multiphoton Microscope

bergamo

Description

A completely modular multiphoton imaging platform that adapts to a wide range of experimental requirements. Featuring five axes of movement including a rotating body, which allows the microscope to rotate around the sample. Equipped with fast resonant scanners and sensitive detectors.

Imaging Mode and Features

Widefield Fluorescence

  • DAPI and GFP LEDs and filter sets.
  • 5MP Monochrome CMOS Kiralux Thorlabs camera.

Laser Scanning 

  • Dual scan paths: Galvo-Galvo (GG) and Galvo-Resonant (GR).
  • (GG): Custom scan geometries and user-defined ROIs for photo stimulation.
  • (GR): 8 kHz Scanner: Image at 2 fps (4096 x 4096 Pixels), 30 fps (512 x 512 Pixels), or 400 fps (512 x 32 Pixels)

Acquisition

  • Deep volumetric imaging (Z-stack), tiling, multipoint, time series.

Detection

  • 2 x High sensitivity GaAsP PMTs.

Objectives 

  • Multiphoton: Nikon LWD 3.0 mm 16x/0.80 NA water.
  • Multiphoton: Nikon Apo LWD 2.0 mm 25x/1.10 NA water.
  • Can accept any Infinity-corrected objectives with M34 x 1.0, M32 x 0.75, M25 x 0.75, or RMS threads (with an adapter).

Lasers

Coherent Chameleon Ultra II Ti:Sapphire tunable femtosecond pulsed laser (680 - 1080 nm).

Additional Features

  • Piezo objective scanner: Enables objective positioning and rapid Z-Stack acquisition with Resolution ~1 nm.
  • Rotation: 0° to 90° around the Sample.

Software
ThorImage®LS 4.3