Tissue Clearing

Turning opaque samples clear for whole organ, deep optical imaging.

Tissue Clearing

Tissue clearing is a form of sample preparation that renders large pieces of biological tissue (eg., whole organs) optically transparent. This provides several advantages:

  • Accurate and uninterrupted 3D analysis of structures in entire organs
  • Can be imaged quickly using lightsheet microscopy
  • Avoid time-consuming tissue sectioning

There are many different variations of tissue clearing (eg., uDISCO, iDISCO, CLARITY, CUBIC) and Expansion Microscopy techniques, in the Advanced Microscopy Platform we provide consultation to help investigators find the technique that is most appropriate for their research needs.

We also provide a range of equipment that is required for implementing these sample preparation techniques, including:

  • LifeCanvas SmartClear Pro II -  High throughput CLARITY device (More info)
  • Temperature controlled incubator
  • Vacuum chamber
  • Benchtop Fume Hood

A review of current techniques (up to 2015):
Richardson, D. S.; Lichtman, J. W. Clarifying Tissue Clearing. Cell 2015, 162 (2), 246–257. https://doi.org/10.1016/j.cell.2015.06.067.

For more information please contact the HBIAMP team.

smartclear

LifeCanvas SmartClear Pro II

cleared brains