Confocal Microscopes - Spectral Imaging
These microscopes are designed for imaging fluorescent samples with high resolution, high contrast, high sensitivity, 3D optical/Z-sectioning.
Additionally they also feature spectral capabilities, using either multi-anode arrays for pixel-by-pixel spectral voxels or prism/slit system to fine tune emission detection.
Leica STELLARIS 5
Features
High throughput Widefield to Confocal imaging pipeline. NIR excitation and detection. TauSense lifetime based contrast and dye separation.
Coming soon!!
Imaging Modes
Inverted DMI8 Platform
Brightfield Transmitted Light and DIC (eyepiece)
Widefield Fluorescence (DAPI, FITC, TRITC, Cy5, mCherry, CFP, YFP, Cy7)
Camera: Leica K8 sCMOS 4.2MP 95fps
LED8 Light engine with 395, 438, 475, 511, 555, 575, 635, 730 nm discrete outputs
Confocal Laser Scanning: X2Y linear Galvo-Galvo scanner
Acquisition: Widefield/Confocal Tiling & Stitching, Z-stack, multi-position, timelapse, lambda, unmixing
Detectors (5 x tuneable channels)
Detection range from 400 - 850 nm
5 x Power HyD S: Si-based, all round performance, high-efficiency in blue/green
Lasers
405 nm diode laser
Tuneable White Light Laser 485 - 790 nm (1 nm increments). Variable Rep Rate
Objectives
2.5x/0.07NA dry; 5x/0.15NA dry; 10x/0.4NA dry; 20x/0.75NA dry; 63x/1.4 NA Oil;
Software
Leica STELLARIS Control Software - LASX Modules
3D Visualisation; Lightning Expert; Navigator Expert; Dye Finder; Assay Editor; Microlab, TauSense
Leica TCS SP8
Features:
Tiling and Stitching, Multi-point, Multiplexing
Imaging Modes
Inverted DMI8 Platform
Brightfield Transmitted Light (eyepiece)
Widefield Fluorescence (eyepiece): DAPI, FITC, Rhodamine
Confocal Laser Scanning (X2Y linear Galvo scanner)
Acquisition: tiling & stitching, z-stack, multiposition, timelapse, lambda scans, unmixing
Detectors (4 x tuneable channels)
Variable filters 380 - 800 nm
2 x low dark current Hamamatsu PMT detectors
2 x high sensitivity hybrid detectors (photon counting mode available)
Lasers
405 nm (DAPI); 488 nm (GFP); 552 nm (Cy3); 640 nm (Alexa 647)
Objectives
5x/0.15NA dry; 10x/0.40 NA dry; 25x/0.95 NA water; 63x/1.30 NA glycerol
Software
Leica Application Suite X (download LASX Core viewer)
Standard Operating Procedure
Download Leica_TCS_SP8_sop
Nikon A1R Confocal Microscope
Features:
32-Channel Spectral Imaging, 8 KHz Resonant Scanner, Upright Platform
Imaging Modes
Upright FN1 Platform
Widefield Fluorescence: DAPI, GFP, and DSRed
Confocal Laser Scanning: Galvo-Galvo or high speed 8 KHz Galvo-Resonant scan mode
Acquisition: z-stack, tiling & stitching, spectral unmixing, multipoint, timelapse
Detection
4 channel PMT detectors: 2 x High Sensitivity GaAsP (green/red); 2 x Multi-Alkali (blue/far-red)
32 channel spectral Multi-Anode detector: 400-750 nm visible range; 2.5/5/10 nm variable resolution
Lasers
405 nm (DAPI); 457, 477, 488, 514 nm (GFP, YFP, FITC, Alexa488); 561 nm (Cy3); 640 nm (Cy5, Alexa 647)
Objectives
10x/0.45 NA dry; 20x/0.75 NA multi-immersion; 60x/1.27 NA water; 16x/0.80 NA water; 25x/1.10 NA water; 60x/1.4 NA oil
Common filter combinations
Blue, Green, Red, Far Red: 450/50 - 525/50 - 600/50 - 685/70 nm
Software
NIS-Elements AR v5.42.01 + offline analysis workstation
Download NIS-Elements viewer
Standard Operating Procedure
Download Nikon_A1R_sop
Nikon C1si Spectral Confocal Microscope
Features
32-Channel Spectral Imaging
Imaging Modes
Inverted Ti-E Platform
Transmitted Light (eyepiece): Brightfield and DIC
Widefield Fluorescence (eyepiece): DAPI, GFP, and TxRed
Confocal Laser Scanning (Galvo-Galvo scanner)
Focus Drift Compensation via Perfect Focus System (PFS)
Acquisition: z-stack, spectral unmixing, multipoint, timelapse
Detection
3 channel standard PMT detectors & 1 channel DIC transmitted light detector
32 channel spectral Multi-Anode detector: 400-750 nm visible range; 2.5/5/10 nm variable resolution
Lasers
405 nm (DAPI), 457, 477, 488, 514 nm (GFP, YFP, FITC, Alexa488), 561 nm (Cy3, Alexa 546/568/594, mCherry); 640 nm (Cy5, Alexa 647)
Objectives
10x/0.45 NA air; 20x/0.75 NA air; 40x/1.30 NA oil; 60x/1.20 NA water
Common Filter Combinations
Blue, Green, Red: 450/35 - 525/50 - 595/50 or 605/60
Green, Red, Far Red: 525/50 - 595/50 or 605/60 - 685/70
Software
EZ-C1 (NIS-Elements AR offline analysis; download EZ-C1 viewer)