Lightsheet Microscope

Selective plane illumination fluorescence for fast and gentle live or optically cleared whole sample imaging.

LaVision BioTec UltraMicroscope II

Lightsheet

Imaging Modes and Features

Fast 3D imaging of large cleared samples
Size up to 30 mm x 30 mm x 15 mm; FOV up to 17.6 mm diagonal; Zoom 1.26x to 12.6x
Compatible with aqueous buffers and organic solvents
Compatible clearing protocols: Scale, SeeDB, CLARITY, DISCO, BABB, CUBIC


Gentle live / In-vivo 3D imaging with environmental control

Available in two interchangeable configurations: SuperPlan and ZoomBody

SuperPlan (default configuration):
Infinity corrected optics provide compatibility with a wide range of objectives. Automated magnification changer (0.6x, 1.0x, 1.67x, 2.5x).
Objectives
MI PLAN 4x/0.35 NA 16.0 mm WD with RI 1.56 corrected dipping cap.
Olympus XLSLPLN25XGMP 25X/1.0 NA 8.0 mm WD with correction collar for RI 1.41 to 1.52

 

ZoomBody (original configuration):
Magnification range from 1.2x to 12.6x
Objective
Olympus MVPLAPO 2x/0.50 NA with 5.7 (RI 1.56 corrected), 6 and 10 (RI 1.33 corrected) mm WD dipping caps

 

Lasers
405 nm (DAPI); 488 nm (GFP); 561 nm (Cy3); 640 nm (Alexa 647); 785 nm (Far Red dyes)

Filters
Blue: 460/40 nm; Green: 525/50 nm; Red: 595/50 nm; Far Red: 670/50 nm; Near Infrared: 805/LP nm

Camera
PCO Edge 4.2 sCMOS camera 

Software
LaVision BioTec ImSpector

Standard Operating Procedure
Download LVBT_UltramicroscopeII_sop