Lightsheet Microscope
Selective plane illumination fluorescence for fast and gentle live or optically cleared whole sample imaging.
LaVision BioTec UltraMicroscope II
Imaging Modes and Features
Fast 3D imaging of large cleared samples
Size up to 30 mm x 30 mm x 15 mm; FOV up to 17.6 mm diagonal; Zoom 1.26x to 12.6x
Compatible with aqueous buffers and organic solvents
Compatible clearing protocols: Scale, SeeDB, CLARITY, DISCO, BABB, CUBIC
Gentle live / In-vivo 3D imaging with environmental control
Available in two interchangeable configurations: SuperPlan and ZoomBody
SuperPlan (default configuration):
Infinity corrected optics provide compatibility with a wide range of objectives. Automated magnification changer (0.6x, 1.0x, 1.67x, 2.5x).
Objectives
MI PLAN 4x/0.35 NA 16.0 mm WD with RI 1.56 corrected dipping cap.
Olympus XLSLPLN25XGMP 25X/1.0 NA 8.0 mm WD with correction collar for RI 1.41 to 1.52
ZoomBody (original configuration):
Magnification range from 1.2x to 12.6x
Objective
Olympus MVPLAPO 2x/0.50 NA with 5.7 (RI 1.56 corrected), 6 and 10 (RI 1.33 corrected) mm WD dipping caps
Lasers
405 nm (DAPI); 488 nm (GFP); 561 nm (Cy3); 640 nm (Alexa 647); 785 nm (Far Red dyes)
Filters
Blue: 460/40 nm; Green: 525/50 nm; Red: 595/50 nm; Far Red: 670/50 nm; Near Infrared: 805/LP nm
Camera
PCO Edge 4.2 sCMOS camera
Software
LaVision BioTec ImSpector
Standard Operating Procedure
Download LVBT_UltramicroscopeII_sop