Lightsheet Microscope
Selective plane illumination fluorescence for fast and gentle live or optically cleared whole sample imaging.
LaVision BioTec UltraMicroscope II
![Lightsheet](/sites/default/files/styles/ucws_image_desktop/public/2019-07/lightsheet.jpg?itok=0bUQy772)
Imaging Modes and Features
Fast 3D imaging of large cleared samples
Size up to 30 mm x 30 mm x 15 mm; FOV up to 17.6 mm diagonal; Zoom 1.26x to 12.6x
Compatible with aqueous buffers and organic solvents
Compatible clearing protocols: Scale, SeeDB, CLARITY, DISCO, BABB, CUBIC
Gentle live / In-vivo 3D imaging with environmental control
Available in two interchangeable configurations: SuperPlan and ZoomBody
SuperPlan (default configuration):
Infinity corrected optics provide compatibility with a wide range of objectives. Automated magnification changer (0.6x, 1.0x, 1.67x, 2.5x).
Objectives
MI PLAN 4x/0.35 NA 16.0 mm WD with RI 1.56 corrected dipping cap.
Olympus XLSLPLN25XGMP 25X/1.0 NA 8.0 mm WD with correction collar for RI 1.41 to 1.52
ZoomBody (original configuration):
Magnification range from 1.2x to 12.6x
Objective
Olympus MVPLAPO 2x/0.50 NA with 5.7 (RI 1.56 corrected), 6 and 10 (RI 1.33 corrected) mm WD dipping caps
Lasers
405 nm (DAPI); 488 nm (GFP); 561 nm (Cy3); 640 nm (Alexa 647); 785 nm (Far Red dyes)
Filters
Blue: 460/40 nm; Green: 525/50 nm; Red: 595/50 nm; Far Red: 670/50 nm; Near Infrared: 805/LP nm
Camera
PCO Edge 4.2 sCMOS camera
Software
LaVision BioTec ImSpector
Standard Operating Procedure
Download LVBT_UltramicroscopeII_sop