Widefield Microscopes

Imaging Modes and Features
Brightfield, Phase Contrast, DIC (60x only)​​​​​​
Fluorescence: DAPI, GFP, TxRed, Cy5
Live Cell Incubator: temperature, humidity, gas control

Objectives
10x/0.30 NA Ph1; 20x/0.45 NA Ph1; 40x/0.60 NA Ph2; 60x/0.70 NA Ph2; 60x/1.30 NA silicon DIC

Filters
DAPI - ex 377/50 nm, em 477/60 nm; GFP - ex 472/30 nm, em 520/35 nm
TxRed - ex 562/40 nm, em 624/40 nm; Cy5 - ex 628/40 nm, em 692/40 nm

Camera
Photometrics monochrome CCD

Software
MetaMorph Advanced Image Acquisition

Imaging Modes
Brightfield, Phase contrast
Fluorescence: DAPI, GFP, Cy3

Objectives
5x/0.15 Ph1; 10x/0.25 Ph1; 20x/0.35 Ph1; 40x/0.55 Ph1

Filters
DAPI - ex 365/12 nm, em LP397 nm; GFP - ex 470/40 nm, em 525/50 nm; Cy3 - ex 545/25 nm, em 605/70 nm

Camera
Axiocam monochrome camera

Software
ZEN

Standard Operating Procedure
Download Zeiss_Axiovert_sop

Imaging Modes
Brightfield, Phase Contrast
Fluorescence and Zeiss Apotome.2 Structured Illumination: DAPI, GFP, and Cy3

Primarily used for Laser microdissection (see link below)

Objectives
5x/0.25; 20x/0.4; 40x/0.6; 100x/1.3 oil

Filters
DAPI ex G365 nm , em 445/50 nm; GFP ex 470/40, em 525/50 nm; Cy3 ex 550/25 nm, em 605/70 nm

Cameras
AxioCam Icc; AxioCam MRm

Software
ZEN

Standard Operating Procedure
Download Zeiss PALM sop

Brightfield or widefield fluorescence high throughput, whole slide scanners (see link below)

Olympus VS110 (5-slide)
Olympus VS120 (100-slide)