Fluorescence Lifetime Imaging Microscopy
Imaging technique based on differences in exponential decay rates of fluorescent probes from a sample featuring video-rate speeds with pixel-by-pixel quantification
Leica SP8 Falcon (FAst Lifetime CONtrast)
This is an advanced microscope available only upon request. Please contact us for more information.
Imaging Modes and Features
Brightfield Transmitted Light and DIC (eyepiece)
Widefield Fluorescence (eyepiece): DAPI, FITC, Rhodamine
Confocal Laser Scanning:
X2Y linear Galvo scanner and X2Y 12kHz Resonant scanner (both with scan rotation)
Super Z-Galvo stage (fast Z-stack)
Acquisition: tiling & stitching, z-stack, multiposition, timelapse, lambda scans, unmixing
5 x Tunable detection channels
Variable filters 380 - 800 nm
1 x low dark current Hamamatsu PMT detectors
2 x high sensitivity hybrid detectors (photon counting mode available)
2 x cooled high sensitivity, single molecule detection (SMD) hybrid detectors (optimized from FLIM)
Objectives
2.5x/0.07NA air; 5x/0.15NA air; 10x/0.40 NA air; 20x/0.8 NA air; 25x/0.95 NA water; 63x/1.40NA oil
Lasers
405 nm diode laser
440 nm pulsed diode laser
Tunable White Light Laser 470 - 670 nm (1nm increments) 10 - 80 MHz Rep Rate using Acousto-Optical Beam Splitter (AOBS)
Software
Leica Application Suite X (download LASX Core viewer)
FALCON FLIM Module with Phasor FLIM Analysis
Standard Operating Procedure
Download Leica_TCS_SP8_FALCON_SOP
Instrument Workstation
Intel 6134 XEON 3.2GHz (8 Core)
192GB RAM
NVIDIA Quadro GP100 16GB Reference GPU (3584 Cuda Cores)
256GB SATA SSD
6TB SATA Enterprise
2TB M.2 SSD
10 GBit INTEL X710 Network Adapter
Windows10 loT Enterprise
Offline Analysis Workstation
HP Z440 Workstation
Intel(R) Xeon(R) CPU E5-1620 v4 @ 3.50GHz, 3501 Mhz, 4 Core(s)
16.0 GB RAM
NVIDIA Quadro P2000 (1024 CUDA cores)
Win 10 Enterprise 2016 LTSB
LASX (Stellaris) with Single Molecule Analysis and Phasor packages