Laser Scanning Confocal Microscopes
Fluorescence imaging with high resolution, high contrast, high sensitivity and 3D optical/Z-sectioning
Leica TCS SP8
Imaging Modes and Features
Brightfield Transmitted Light (eyepiece)
Widefield Fluorescence (eyepiece): DAPI, FITC, Rhodamine
Confocal Laser Scanning (X2Y linear Galvo scanner)
Acquisition: tiling & stitching, z-stack, multiposition, timelapse, lambda scans, unmixing
4 x Tunable detection channels
Variable filters 380 - 800 nm
2 x low dark current Hamamatsu PMT detectors
2 x high sensitivity hybrid detectors (photon counting mode available)
Objectives
5x/0.15NA air; 10x/0.40 NA air; 25x / 0.95 NA water; 63x/1.30 NA glycerol
Lasers
405 nm (DAPI); 488 nm (GFP); 552 nm (Cy3); 640 nm (Alexa 647)
Software
Leica Application Suite X (download LASX Core viewer)
Standard Operating Procedure
Download Leica_TCS_SP8_sop
ALSO AVAILABLE
Time-correlated single-photon counting (TCSPC) Fluorescence Lifetime Leica SP8 FALCON equipped with white light laser. Please contact us to discuss project details and access.
Nikon A1R Confocal Microscope
Imaging Modes and Features
Widefield Fluorescence: DAPI, GFP, and DSRed
Confocal Laser Scanning: Galvo-Galvo or high speed Galvo-Resonant scan modes
Acquisition: z-stack, tiling & stitching, spectral unmixing, multipoint, timelapse
Detection
4 channel PMT detectors: 2 x High Sensitivity GaAsP (green/red); 2 x Multi-Alkali (blue/far-red)
32 channel spectral Multi-Anode detector: 400-750 nm visible range; 2.5/5/10 nm variable resolution
Objectives
10x/0.45 NA air; 20x/0.75 NA multi-immersion; 60x/1.27 NA water; 16x/0.80 NA water; 25x/1.10 NA water; 60x/1.4 NA oil
Lasers
405 nm (DAPI); 457, 477, 488, 514 nm (GFP, YFP, FITC, Alexa488); 561 nm (Cy3); 640 nm (Cy5, Alexa 647)
Common filter combinations
Blue, Green, Red, Far Red: 450/50 - 525/50 - 600/50 - 685/70 nm
Software
NIS-Elements AR v5.42.01 + offline analysis workstation
Download NIS-Elements viewer
Standard Operating Procedure
Download Nikon_A1mp_sop
Nikon C1si Spectral Confocal Microscope
Imaging Modes and Features
Transmitted Light (eyepiece): Brightfield and DIC
Widefield Fluorescence (eyepiece): DAPI, GFP, and TxRed
Confocal Laser Scanning (Galvo-Galvo scanner)
Focus Drift Compensation via Perfect Focus System (PFS)
Acquisition: z-stack, spectral unmixing, multipoint, timelapse
Detection
3 channel standard PMT detectors & 1 channel DIC transmitted light detector
32 channel spectral Multi-Anode detector: 400-750 nm visible range; 2.5/5/10 nm variable resolution
Objectives
10x/0.45 NA air; 20x/0.75 NA air; 40x/1.30 NA oil; 60x/1.20 NA water
Lasers
405 nm (DAPI), 457, 477, 488, 514 nm (GFP, YFP, FITC, Alexa488), 561 nm (Cy3, Alexa 546/568/594, mCherry); 640 nm (Cy5, Alexa 647)
Common Filter Combinations
Blue, Green, Red: 450/35 - 525/50 - 595/50 or 605/60
Green, Red, Far Red: 525/50 - 595/50 or 605/60 - 685/70
Software
EZ-C1 (NIS-Elements AR offline analysis; download EZ-C1 viewer)