Laser Scanning Confocal Microscopes
Fluorescence imaging with high resolution, high contrast, high sensitivity and 3D optical/Z-sectioning
Leica TCS SP8
Imaging Modes and Features
Brightfield Transmitted Light (eyepiece)
Widefield Fluorescence (eyepiece): DAPI, FITC, Rhodamine
Confocal Laser Scanning (X2Y linear Galvo scanner)
Acquisition: tiling & stitching, z-stack, multiposition, timelapse, lambda scans, unmixing
4 x Tunable detection channels
Variable filters 380 - 800 nm
2 x low dark current Hamamatsu PMT detectors
2 x high sensitivity hybrid detectors (photon counting mode available)
Objectives
5x/0.15NA air; 10x/0.40 NA air; 25x / 0.95 NA water; 63x/1.30 NA glycerol
Lasers
405 nm (DAPI); 488 nm (GFP); 552 nm (Cy3); 640 nm (Alexa 647)
Software
Leica Application Suite X (download LASX Core viewer)
Standard Operating Procedure
Download Leica_TCS_SP8_sop
ALSO AVAILABLE
Time-correlated single-photon counting (TCSPC) Fluorescence Lifetime Leica SP8 FALCON equipped with white light laser. Please contact us to discuss project details and access.
Nikon A1R-MP Multiphoton Microscope
Imaging Modes and Features
Widefield Fluorescence: DAPI, GFP, and DSRed
Confocal Laser Scanning: Galvo-Galvo or high speed Galvo-Resonant scan modes
Acquisition: z-stack, tiling & stitching, spectral unmixing, multipoint, timelapse
This is also a Multiphoton microscope (see link below)
Detection
4 channel PMT detectors: 2 x High Sensitivity GaAsP (green/red); 2 x Multi-Alkali (blue/far-red)
32 channel spectral Multi-Anode detector: 400-750 nm visible range; 2.5/5/10 nm variable resolution
Objectives
10x/0.45 NA air; 20x/0.75 NA multi-immersion; 60x/1.27 NA water; 16x/0.80 NA water; 25x/1.10 NA water; 60x/1.4 NA oil
Lasers
405 nm (DAPI); 457, 477, 488, 514 nm (GFP, YFP, FITC, Alexa488); 561 nm (Cy3); 640 nm (Cy5, Alexa 647)
Common filter combinations
Blue, Green, Red, Far Red: 450/50 - 525/50 - 600/50 - 685/70 nm
Software
NIS-Elements AR v5.42.01 + offline analysis workstation
Download NIS-Elements viewer
Standard Operating Procedure
Download Nikon_A1mp_sop
Nikon C1si Spectral Confocal Microscope
Imaging Modes and Features
Transmitted Light (eyepiece): Brightfield and DIC
Widefield Fluorescence (eyepiece): DAPI, GFP, and TxRed
Confocal Laser Scanning (Galvo-Galvo scanner)
Focus Drift Compensation via Perfect Focus System (PFS)
Acquisition: z-stack, spectral unmixing, multipoint, timelapse
Detection
3 channel standard PMT detectors & 1 channel DIC transmitted light detector
32 channel spectral Multi-Anode detector: 400-750 nm visible range; 2.5/5/10 nm variable resolution
Objectives
10x/0.45 NA air; 20x/0.75 NA air; 40x/1.30 NA oil; 60x/1.20 NA water
Lasers
405 nm (DAPI), 457, 477, 488, 514 nm (GFP, YFP, FITC, Alexa488), 561 nm (Cy3, Alexa 546/568/594, mCherry); 640 nm (Cy5, Alexa 647)
Common Filter Combinations
Blue, Green, Red: 450/35 - 525/50 - 595/50 or 605/60
Green, Red, Far Red: 525/50 - 595/50 or 605/60 - 685/70
Software
EZ-C1 (NIS-Elements AR offline analysis; download EZ-C1 viewer)