Laser Scanning Confocal Microscopes

Fluorescence imaging with high resolution, high contrast, high sensitivity and 3D optical/Z-sectioning

Leica TCS SP8

SP8

Imaging Modes and Features
Brightfield Transmitted Light (eyepiece)
Widefield Fluorescence (eyepiece): DAPI, FITC, Rhodamine
Confocal Laser Scanning (X2Y linear Galvo scanner)
Acquisition: tiling & stitching, z-stack, multiposition, timelapse, lambda scans, unmixing

4 x Tunable detection channels
Variable filters 380 - 800 nm
2 x low dark current Hamamatsu PMT detectors
2 x high sensitivity hybrid detectors (photon counting mode available)

Objectives
5x/0.15NA air; 10x/0.40 NA air; 25x / 0.95 NA water; 63x/1.30 NA glycerol

Lasers
405 nm (DAPI); 488 nm (GFP); 552 nm (Cy3); 640 nm (Alexa 647)

Software
Leica Application Suite X (download LASX Core viewer)

Standard Operating Procedure
Download Leica_TCS_SP8_sop

ALSO AVAILABLE
Time-correlated single-photon counting (TCSPC) Fluorescence Lifetime Leica SP8 FALCON equipped with white light laser. Please contact us to discuss project details and access.

Nikon A1R Confocal Microscope

A1

Imaging Modes and Features
Widefield Fluorescence: DAPI, GFP, and DSRed
Confocal Laser Scanning: Galvo-Galvo or high speed Galvo-Resonant scan modes 
Acquisition: z-stack, tiling & stitching, spectral unmixing, multipoint, timelapse

Detection
4 channel PMT detectors: 2 x High Sensitivity GaAsP (green/red); 2 x Multi-Alkali (blue/far-red)
32 channel spectral Multi-Anode detector: 400-750 nm visible range; 2.5/5/10 nm variable resolution

Objectives 
10x/0.45 NA air; 20x/0.75 NA multi-immersion; 60x/1.27 NA water; 16x/0.80 NA water; 25x/1.10 NA water; 60x/1.4 NA oil

Lasers 
405 nm (DAPI); 457, 477, 488, 514 nm (GFP, YFP, FITC, Alexa488); 561 nm (Cy3); 640 nm (Cy5, Alexa 647)

Common filter combinations 
Blue, Green, Red, Far Red: 450/50 - 525/50 - 600/50 - 685/70 nm

Software
NIS-Elements AR v5.42.01 + offline analysis workstation
Download NIS-Elements viewer

Standard Operating Procedure
Download Nikon_A1mp_sop

Nikon C1si Spectral Confocal Microscope

C1si

Imaging Modes and Features
Transmitted Light (eyepiece): Brightfield and DIC
Widefield Fluorescence (eyepiece): DAPI, GFP, and TxRed
Confocal Laser Scanning (Galvo-Galvo scanner)
Focus Drift Compensation via Perfect Focus System (PFS)
Acquisition: z-stack, spectral unmixing, multipoint, timelapse

Detection
3 channel standard PMT detectors & 1 channel DIC transmitted light detector
32 channel spectral Multi-Anode detector: 400-750 nm visible range; 2.5/5/10 nm variable resolution

Objectives
10x/0.45 NA air; 20x/0.75 NA air; 40x/1.30 NA oil; 60x/1.20 NA water

Lasers
405 nm (DAPI), 457, 477, 488, 514 nm (GFP, YFP, FITC, Alexa488), 561 nm (Cy3, Alexa 546/568/594, mCherry); 640 nm (Cy5, Alexa 647)

Common Filter Combinations
Blue, Green, Red: 450/35 - 525/50 - 595/50 or 605/60
Green, Red, Far Red: 525/50 - 595/50 or 605/60 - 685/70

Software
EZ-C1 (NIS-Elements AR offline analysis; download EZ-C1 viewer)